Measuring nitrate with the colorimeter

API nitrate test kit

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API nitrate test kit

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Reagents & Equipment

  • Colorimeter
  • Cuvettes
  • 5 mL sample bottles or other capped tubes
  • API nitrate test kit
  • (Optional) Distilled water for sample dilutions

Colorimeter set-up

  • Wavelength: 528 nm. Green led (colorimeter RGB board)
  • Nitrate standard data: download a data file or prepare your own nitrate standard data
  • Before measuring blank the colorimeter against water or a distilled water sample developed with API reagent as described below

Method

  • Fill an empty sample bottle to the 5 mL line with your water sample.
  • Add 10 drops of reagent from Bottle # 1 to the sample, cap and invert several times to mix
  • Vigorously shake Bottle # 2 for 30-60 secs
  • Add 10 drops of reagent from Bottle # 2
  • Cap the bottle and shake thoroughly to mix.
  • Wait at least 5 mins for the color to develop.
  • Pour some of the sample into a cuvette, place in the colorimeter and click measure. The program will return the nitrate concentration in ppm.

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