Measuring ammonia with the colorimeter

API ammonia test kit

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API ammonia test kit

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Reagents & Equipment

  • Colorimeter
  • Cuvettes
  • 5 mL sample bottles or other capped tubes
  • API ammonia test kit
  • (Optional) Distilled water for sample dilutions

Colorimeter set-up

  • Wavelength: 625 nm. Red led (colorimeter RGB board)
  • Ammonia standard data: download a data file or prepare your own nitrate standard data
  • Before measuring blank the colorimeter against water or a distilled water sample developed with API reagent as described below

Method

  • Fill an empty sample bottle to the 5 mL line with your water sample.
  • Add 8 drops of API Ammonia Test Solution, Bottle #1.
  • Cap the bottle and invert to mix the solutions.
  • Add 8 drops of API Ammonia Test Solution, Bottle # 2.
  • Cap the bottle and shake thoroughly to mix.
  • Wait 5-10 mins for the color to fully develop.
  • Pour some of the sample into a cuvette, place in the colorimeter and click measure. The program will return the ammonia concentration in ppm.

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